P.3.008 S-Adenosyl-methionine impairs forced swimming-induced behavioural immobility by inhibiting gene expression in dentate gyrus neurons

Results: As compared to the CNT fibroblasts (75 affected microRNAs), the D samples showed almost three times more microRNA expression changes (214 microRNAs) caused by both GAL and NL metabolic stress treatments. The microRNA profile alterations were more remarkable under NL circumstances. Although higher overlap was detected between the GAL and NL induced microRNA changes in the D group (47 microRNAs, while only 4 microRNAs in the CNT), Pearson correlation showed that the two different stress treatments resulted in very similar microRNA expression pattern in the CNT (r2 = 0.51 and 0.43) but not in the D group. Nearly 10% of the suspected target mRNAs of the most significantly affected microRNAs changed due to metabolic stress treatments in the CNT group. Conclusion: We suppose that the metabolic stress induced microRNA changes in dermal fibroblasts of CNT subjects are part of the normal cell adaptation, while those found in the D group could be the sign of a genetically determined, pathologic stress response presumed in depression. These results could facilitate the understanding of microRNAs’ role in the development of depression and contribute to the mapping of the stress related physiologic and degenerative gene expression changes by setting up a potential new in vitro metabolic stress related depression model and diagnostic tool.